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MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from pwn_comment where pid='677212' and iffb='1') called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='677212' and iffb='1') called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:65] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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Warning: mysql_query() [function.mysql-query]: Unable to save result set in D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php on line 50
Database error: Invalid SQL: select * from pwn_comment where pid='677212' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='677212' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='677212' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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网友点评-N HCC cells.L.obtusiloba extract decreases transcriptional activity of NF-BThe-线缆测高仪,超声波测高仪, 手持式测高仪-上海交通大学科技园上海野豹企业发展公司
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发布于:2018-12-6 22:27:10  访问:18 次 回复: 篇
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N HCC cells.L.obtusiloba extract decreases transcriptional activity of NF-BThe
Since L.obtusiloba extract induces apoptosis (Salvianolic acid B manufacturer Figure 1B) and displays anti-inflammatory activity [32], we assessed whether the extract decreasesTable 2 Danirixin site Expression of PPARg, COX-2 and iNOS in human HCC cell linesPPARg expression IGF-1 L. As described for Table 2, HCC cells treated for 24 h with 100 g/ml L.obtusiloba extract, 125 ng/ml IGF-1, a combination of both or untreated cells were lysed. Whole cell lysates were analyzed by western-blot with antibodies specific for phosphorylated and normal IGF-1R and its downstream targets Akt and Stat3. Bands were visualized by chemiluminescence. Blots are representative for three independent experiments. Quantification of the protein-activation (nfold to control values) were taken from table 3.the activity of NF-B in HCC cells (Figure 3). All four HCC cell lines transfected for transient constitutive expression of NF-B exhibited high levels of basal NFB transcriptional activity of about 160 260 RLU. This activity was not significantly increased by addition of TNFa. In all cell lines, treatment of transfected cells with the specific NF-B-inhibitor 17-DMAG reduced the activity to <10 of the basal level thus approving the function of the experimental system (data not shown). Except for HepG2 cells, L.obtusiloba extract attenuated the transcriptional activity of NF-B to 75(P < 0.05) of the basal level in Huh-7 and to 65 (P < 0.001) in Hep3B cells while in the poorly differentiated SK-Hep1 cells the high basal transcriptional activity of NF-B was reduced to 50 (P < 0.001).N HCC cells.L.obtusiloba extract decreases transcriptional activity of NF-BThe IGF-1/IGF-1R axis plays an important role in angiogenesis and therefore the development of HCC. To PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28607003 investigate signaling pathways involved, western-blots specific for (p)IGF-1R and the activation states of its target proteins were focused on Hep3B as one out of the three less invasive HCC cells (compare Figure 1C) and the more aggressive SK-Hep1 cells. In both cell linesNF-B is a key regulator of crucial pro-inflammatory cytokines during carcinogenesis and promotes cell survival and angiogenesis. Since L.obtusiloba extract induces apoptosis (Figure 1B) and displays anti-inflammatory activity [32], we assessed whether the extract decreasesTable 2 Expression of PPARg, COX-2 and iNOS in human HCC cell linesPPARg expression IGF-1 L. obtusiloba extract 0.76 ?0.14 0.84 ?0.09 0.21 ?0.10* IGF-1 and L. obtusiloba extract 1.15 ?0.09# 0.81 ?0.#COX-2 expression IGF-1 L.obtusiloba extract n.d. 0.77 ?0.08* n.d. 0.80 ?0.07* IGF-1 and L. obtusiloba extract n.d. 1.09 ?0.04*; n.d. 0.82 ?0.09##iNOS expression IGF-1 L. obtusiloba extract 0.21 ?0.14* n.d. n.d. IGF-1 and L. obtusiloba extract 0.21 ?0.09*;# n.d. n.d. 0.35 ?0.06*;#HepG2 4.31 ?0.51* Hep3B 1.33 ?0.12* Huh-7 SKHep1 1.17 ?0.n.d. 2.28 ?0.19* n.d. 3.21 ?0.34*1.17 ?0.07 n.d. n.d.0.31 ?0.12*;# 0.89 ?0.07#1.43 ?0.11* 0.75 ?0.09*1.87 ?0.12* 0.27 ?0.12*Cell lysates of HCC cell lines as described for Table 1, were subjected to specific western-blots for PPARg, COX 2 and iNOS and for b actin as equal loading control.
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