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Database error: Invalid SQL: select * from pwn_comment where pid='635261' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='635261' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='635261' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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Both, 5‘ and 3‘UTR conserved motifs were searched, based on our previous research work [19].Nucleotide sequence accession DMOG web numbersThe 21 new cDNA sequences have been deposited in the GenBank Database. (DOCX 16 kb) Additional file 4: Quantitative RT-PCR standard-curve parameters. (DOCX 18 kb) Additional file 5: Ciliate metallothionein GenBank accession numbers. (DOCX 17 kb)Abbreviations CdMTs: cadmium-binding MTs; CuMTs: copper-binding-MTs; LC50: lethal concentration killing 50 of cell population; MTs: metallothioneins;References 1. Coyle P, Philcox JC, Carey LC, Rofe AM. Metallothionein: the multipurpose protein. Cell Mol Life Sci. 2002;59:627?7. 2. Viarengo A, Burlando B, Ceratto N, Panfoli I. A PhD scholarship was awarded to PdF by the Spanish Ministry of Education (FPU12/02789). Author details 1 Departamento Microbiolog -III, Facultad de Biolog . C/Jos?Antonio Novais, 12, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain. 2 Universidad Castilla-La Mancha, Campus Tecnol ico de la f rica de armas, Edificio Sabatini. Av. Carlos III, s/n. 45071 Toledo, Spain. Received: 17 December 2015 Accepted: 22 AprilDNA sequences were obtained with the ABI Prism 3730 DNA Analyser sequencer (Applied Biosystem). In order to look for new MT sequences we also analyzed the macronuclear genomes of T. malaccensis, T. borealis and T. elliotti with the BLAST program on the Tetrahymena Comparative Database website (http://www.broadinstitute.org/scientific-comunity/science/ programs/genome-sequencing-and-analysis/update-ourmicrobial-eukaryotes) and the macronuclear genome of I. multifiliis with the BLAST program on the Ichthyophthirius Genome Database website (http://ich.ciliate.org/index.php/ home/welcome). Multiple sequence alignments were performed using T-Coffee online application (which uses the Clustal Wallis method) (Tree-based Consistency Objective Function for Alignment Evaluation) [74]. The phylogenetic trees of both Tetrahymena MTs and SSrRNAs were built using the maximum likehood algorithm (MEGA 5.05, using the Poisson model). Phylogenetic trees (Newick) and alignments (FASTA) are available from TreeBase (http://purl.org/phylo/treebase/phylows/ study/TB2:S18773, http://purl.org/phylo/treebase/phylows/ study/TB2:S18776) with references: TB2:S18773 and TB2:S18776. Both, 5‘ and 3‘UTR conserved motifs were searched, based on our previous research work [19].Nucleotide sequence accession numbersThe 21 new cDNA sequences have been deposited in the GenBank Database. All GenBank accession numbers of MT genes referred on this article are shown in Additional file 5.Availability of data and materialsThe datasets supporting the conclusions of this article are included within the article (and its Additional file 5) and are available in the TreeBase repository (http://purl.org/ phylo/treebase/phylows/study) with PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25679764 references: TB2:S18773 and TB2:S18776.Additional filesAdditional file 1: Theoretical metal binding capacity for Tetrahymena Cd- and CuMTs. (DOCX 16 kb) Additional file 2: Heavy metal toxicity analysis. (DOCX 17 kb) Additional file 3: Primers for standard PCR and qRT-PCR. (DOCX 16 kb) Additional file 4: Quantitative RT-PCR standard-curve parameters. (DOCX 18 kb) Additional file 5: Ciliate metallothionein GenBank accession numbers. (DOCX 17 kb)Abbreviations CdMTs: cadmium-binding MTs; CuMTs: copper-binding-MTs; LC50: lethal concentration killing 50 of cell population; MTs: metallothioneins;References 1. Coyle P, Philcox JC, Carey LC, Rofe AM. Metallothionein: the multipurpose protein. Cell Mol Life Sci. 2002;59:627?7. 2. Viarengo A, Burlando B, Ceratto N, Panfoli I. Antioxidant role of metallothioneins: a comparative overview.
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