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MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='598817' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='598817' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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网友点评-Nt cells. MV entry into mouse cells {is also|can also-线缆测高仪,超声波测高仪, 手持式测高仪-上海交通大学科技园上海野豹企业发展公司
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发布于:2018-10-17 23:11:47  访问:76 次 回复: 篇
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Nt cells. MV entry into mouse cells {is also|can also
Respiratory inoculation of these mice with MV-Edm resulted in WST-8 enhanced virus spread and much more prominent lung tissue inflammation, and intracerebral infection was lethal at low virus doses.Supplies AND Solutions CD46 transgenic mice. The outcomes obtained using the three primer pairs have been equivalent. Southern blots probed using the 263-bp PCR product of your CD46 promoter and with a Prn-p gene fragment (5), both nick translated with PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24741339 the Prime-it I.Nt cells. MV entry into mouse cells can also be much more effective
Nt cells. MV entry into mouse cells is also additional efficient with expression of CD46, the receptor for the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21961645 MV vaccine strain Edmonston (MV-Edm) (13, 36) and almost certainly for quite a few wild-type strains (51, 52, 57). However, transgenic rodents expressing CD46 are certainly not susceptible to MV infection when inoculated by the all-natural respiratory route (17, 39, 56). Nevertheless, MV-Edm intracerebral inoculation of neonatal transgenic mice expressing 1 form of CD46 in neurons resulted in disease and death (46). To obtain mice in which MV spread is usually studied, we operated at two levels. First, figuring out that alpha/beta interferon controls MV infection in cultured cells (6, 28) and may perhaps do so in patients with SSPE (15, 60), we tested whether or not mice having a targeted mutation inactivating the alpha/beta interferon receptor (Ifnartm strain) (35) are susceptible to infection with the attenuated strain MV-Edm. Certainly we observed limited MV spread immediately after intranasal inoculation and 50 lethality soon after high-dose intracerebral inoculation. Second, we created the new transgenic line CD46Ge (Ge for genomic), which, unlike earlier lines (17, 46, 56), expresses CD46 with human-like tissue specificity (34, 42), and crossed it with Ifnartm mice to acquire an Ifnartm-CD46Ge line. Respiratory inoculation of these mice with MV-Edm resulted in enhanced virus spread and much more prominent lung tissue inflammation, and intracerebral infection was lethal at low virus doses.Components AND Strategies CD46 transgenic mice. A yeast strain carrying the 400-kb yeast artificial chromosome (YAC) RCA1, using the CD46 gene in its center (18), was grown in selective medium, and YAC DNA was prepared (48). Briefly, yeast cells have been embedded in agarose and lysed, the intact chromosomal DNA was separated by pulsed-field gel electrophoresis, a gel slice using the YAC was identified and excised, plus the DNA was concentrated on a second agarose gel cast around the slice. To compact the DNA and reduce shearing forces, buffers containing NaCl and polyamines have been utilized. After agarase digestion, YAC DNA was dialyzed against microinjection buffer (100 mM NaCl, ten mM Tris-HCl [pH 7.5], 0.1 mM EDTA, 30 M spermine, 70 M spermidine). About 2 pl of purified YAC DNA (2 ng/ l) in microinjection buffer was injected into fertilized oocytes of B6C3F1 hybrids, and about 30 oocytes were transferred to each and every pseudopregnant foster mother. Seventeen pups had been born, of which two have been transgenic. 1 animal (Hugo) was a uncommon mosaic nontransmitter, whereas TgN(hCD46Ge) 373Zbz (Teresa) transmitted its insert to about 50 of its progeny.
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