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MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from pwn_comment where pid='544203' and iffb='1') called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='544203' and iffb='1') called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:65] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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Database error: Invalid SQL: select * from pwn_comment where pid='544203' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='544203' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='544203' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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网友点评-Se reaction on various DNA substrates.-线缆测高仪,超声波测高仪, 手持式测高仪-上海交通大学科技园上海野豹企业发展公司
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发布于:2018-9-17 23:26:25  访问:81 次 回复: 篇
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Se reaction on various DNA substrates.
The Basmisanil References substrates that had been tested were substrate II (A), substrate III (B), and substrate I, which was 6-FAM labeled in the five end of either oligonucleotide 1 (D) or oligonucleotide two (E). This stimulatory effect of RuvAMge on RuvBMge was RuvAMge concentration dependent (Fig. 3E, lanes 7 to ten). Like RuvAMpn and RuvAEco (39), RuvAMge alone was unable to catalyze DNA strand displacement (data not shown).Se reaction on different DNA substrates. The substrates that had been tested were substrate II (A), substrate III (B), and substrate I, which was 6-FAM labeled in the five end of either oligonucleotide 1 (D) or oligonucleotide 2 (E). Reactions had been performed as described within the legend of Fig. 3 and contained either 0 M RuvBFH or 2.7 M RuvBFH within the presence of Mg2 (10 mM) and ATP (2 mM). Samples were taken at the time points indicated above the lanes on the figures. The labeled oligonucleotides 1 and two are taken along as makers in panel A (lane 9) and panel B (lane 9), respectively. (C) Comparison of your percentage of unwinding (the percentage of displaced oligonucleotide) of substrate II (f) with that of substrate III ( ). The displaced oligonucleotides had been measured in the gels shown in panels A and B as the percentage of released solution relative to the total substrate within the reaction mixture. The labeling of substrate and reaction goods is comparable to that used in Fig. 3.stranded oligonucleotide items, will not be recognized. On the other hand, as their formation depended around the presence of unlabeled oligonucleotide 1 inside the substrate, it is most likely that these goods represent incorrectly annealed duplexes of unlabeled oligonucleotide 1, which can be preferentially developed inside the helicase reaction, and labeled oligonucleotide 2. This notion was corroborated by the observation that similar merchandise have been formed soon after labeled oligonucleotide two was mixed with unlabeled oligonucleotide 1 at relatively low temperatures ( 37 ) (data not shown). Like the RuvBFH protein, RuvBMge also displayed DNA unwinding activity. This activity, even so, was substantially reduced than that of its M. pneumoniae ortholog (Fig. 3C, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28077646 lanes two and three). This discovering corresponded with the outcomes in the ATPase assay, in which RuvBMge was discovered to have a reduce activity than RuvBFH (Fig. 1C). The DNA helicase activities of each RuvBFH and RuvBMge are outstanding as the counterpart of those proteins from E. coli, RuvBEco, was previously reported to be incapable of DNA unwinding by itself (39). Although the RuvBEco protein does have intrinsic DNA helicase activity, the protein is capable to exert this activity only within the presence of RuvAEco (39). To investigate regardless of whether the RuvA PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24475050 homologues from M. pneumoniae and M. genitalium (RuvAMpn and RuvAMge, respectively) can influence the activities of RuvBFH and RuvBMge, many concentrations on the RuvA proteins had been incorporated in helicase assays applying a selection of DNA substrates. As shown in Fig. 3D, the helicase activity of RuvBFH on substrate I was not changed substantially by RuvAMpn. We had been also unable to detect asignificant effect of RuvAMpn on RuvBFH activity with other DNA substrates and at diverse RuvBFH concentrations (Fig.
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