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Database error: Invalid SQL: select * from pwn_comment where pid='464666' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='464666' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\includes\db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='464666' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\hs21cn2043\wwwroot\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\hs21cn2043\wwwroot\includes\common.inc.php:551] #3 printpage() called at [D:\wwwroot\hs21cn2043\wwwroot\comment\html\index.php:13]
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网友点评-F iASPP in HeLa cells considerably enhanced the p53 occupancy at-线缆测高仪,超声波测高仪, 手持式测高仪-上海交通大学科技园上海野豹企业发展公司
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发布于:2018-8-2 21:31:01  访问:31 次 回复: 篇
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F iASPP in HeLa cells considerably enhanced the p53 occupancy at
In contrast, SiHa cells transfected with CP-673451 PDGFR Mimic-20a displayed a downregulation of E-cadherin and an induction of Vimentin, compared with handle cells transfected with mimic-NC (Fig. This evaluation revealed that much more than 1000 genes have been up-regulated by miR-20a knockdown. Making use of this list, we performed online miRDB target gene prediction analysis [23], and DMXAA narrowed the predicted targets of miR-20a down to 73 frequent genes (Fig. 4a; Extra file 1: Table S2).GAPDHpXiong et al. Journal of Experimental Clinical Cancer Analysis (2017) 36:Web page 6 ofARelative miR-20a levelBRelative miR-20a level** **6.0 4.0 2.EM SiHa HeLa1.two 0.8 0.4**6.0 four.0 2.0Anti-NC Anti-20a E-cadherin Vimentin GAPDHAnti-20aAnti-NC8.1.Relative miR-20a level8.E-cadherin Vimentin GAPDHAnti-NC + Anti-20a - +Mimic-NC + Mimic-20a - +EHeLaFshRNA-NC + Mimic-NC shRNA-iASPP + Mimic-NC shRNA-iASPP + Mimic-20a Empty vector + Anti-NC iASPP vector + Anti-NC iASPP vector+ Anti-20aGRelative survival0.six 0.4 0.2Relative survival0.HeLa0.8 0.six 0.four 0.2SiHaRelative invasionRelative invasion1.six 1.2 0.8 0.4HeLa**4.0 3.0 2.0 1.0**Mimic-NC****Anti-NCAnti-20aSiHaDDP shRNA-NC shRNA-iASPP Mimic-20a+ + + + + + - - - + + - + - +DDP shRNA-NC shRNA-iASPP Mimic-20a+ + + + + + - - - + + - + - +Fig. 3 miR-20a mediates iASPP-induced EMT and cisplatin resistance.F iASPP in HeLa cells considerably enhanced the p53 occupancy at the miR-20a promoter, although enforced expression of iASPP in SiHa cells triggered a release of p53 from the miR-20a promoter (Fig. 2e and f ). These results indicate that iASPP prevents p53 recruitment to the miR-20a promoter, top to the induction of miR-20a in CC cells.miR-20a mediates iASPP-induced EMT and cisplatin resistancewith adjustments in EMT markers. Western blotting data showed that HeLa cells transfected with anti-20a displayed an upregulation of E-cadherin plus a repression of Vimentin, compared with handle cells transfected with anti-NC (Fig. 3d). Knockdown of miR-20a also resulted in decreased invasiveness of HeLa cells (Fig. 3e). In contrast, SiHa cells transfected with mimic-20a displayed a downregulation of E-cadherin and an induction of Vimentin, compared with handle cells transfected with mimic-NC (Fig. 3b and d). Of note, enforced miR-20a expression partially rescued the effects PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27465830 of iASPP knockdown by sustaining invasiveness of HeLa cells (Fig. 3f), whereas anti-20amediated downregulation of miR-20a drastically attenuated iASPP-driven invasion of SiHa cells (Fig. 3f). CCK8 assay demonstrated that overexpression of miR-20a significantly decreased the activity of cisplatin by restoring cell survival, which was suppressed by iASPP depletion (Fig. 3g). These data recommend that miR-20a is essential for iASPP-induced invasion and cisplatin resistance in CC cells.miR-20a-FBXL5/BTG3 signaling is accountable for iASPPinduced cell invasion and cisplatin resistanceqPCR analysis of CC cell lines and immortalized standard epithelial cell line EM demonstrated the up-regulation of miR-20a in CC cells (Fig. 3a). We subsequent sought to ascertain the function of miR-20a in regulating iASPP-induced PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25112874 EMT and cisplatin resistance by modulating its levels in CC cells.
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